电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

TNC ELISA 试剂盒

TNC 适用: 人 Colorimetric Sandwich ELISA 6.1-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4884835
发货至: 中国
  • 抗原 See all TNC ELISA试剂盒
    TNC (Tenascin C (TNC))
    适用
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    6.1-1500 pg/mL
    最低检测浓度
    6.1 pg/mL
    应用范围
    ELISA
    原理
    Human Tenascin C ELISA Kit for serum, plasma, and cell culture supernatant samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects human Tenascin C. Other species not determined.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling TNC ELISA Kit
    Top Product
    Discover our top product TNC ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples1,000 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all TNC ELISA试剂盒
    TNC (Tenascin C (TNC))
    别名
    Tenascin C (TNC 产品)
    别名
    tn ELISA Kit, 150-225 ELISA Kit, GMEM ELISA Kit, GP ELISA Kit, HXB ELISA Kit, JI ELISA Kit, TN ELISA Kit, TN-C ELISA Kit, AI528729 ELISA Kit, C130033P17Rik ELISA Kit, Hxb ELISA Kit, Ten ELISA Kit, cytotactin ELISA Kit, tenascin-C ELISA Kit, P230 ELISA Kit, tenc ELISA Kit, wu:fk04d02 ELISA Kit, tenascin C L homeolog ELISA Kit, tenascin C ELISA Kit, tnc.L ELISA Kit, TNC ELISA Kit, MCYG_00667 ELISA Kit, tnc ELISA Kit, Tnc ELISA Kit
    基因ID
    3371
    UniProt
    P24821
    途径
    Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
You are here:
客服