TNC ELISA 试剂盒
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Quick Overview for TNC ELISA 试剂盒 (ABIN2859318)
抗原
See all TNC ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.7 pg/mL
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原理
- Sandwich Human Tenascin-C/TNC ELISA Kit to quantitate Human TNC in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G23-P625Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Tenascin C (TNC) ELISA Kit
TNC 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 小鼠 Colorimetric Competition ELISA 9.4 pg/mL - 600 pg/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.8%, 7.1%, 7.4%
Inter-Assay Precision (CV%): 6.2%, 8.3%, 7.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- TNC (Tenascin C (TNC))
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别名
- TNC
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背景
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Background: Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest, it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.
Gene Full Name: tenascin C
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基因ID
- 3371
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UniProt
- P24821
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途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
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