SFRP1 ELISA 试剂盒
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- 抗原 See all SFRP1 ELISA试剂盒
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Human sFRP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human sFRP-1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling SFRP1 ELISA Kit
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- Discover our top product SFRP1 ELISA Kit
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Secreted Frizzled-Related Protein 1 (SFRP1) ELISA Kit
SFRP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 1 (SFRP1) ELISA KitSFRP1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 1 (SFRP1) ELISA KitSFRP1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted Frizzled-Related Protein 1 (SFRP1) ELISA KitSFRP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples3 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all SFRP1 ELISA试剂盒
- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
- 别名
- sFRP-1 (SFRP1 产品)
- 别名
- FRP ELISA Kit, FRP-1 ELISA Kit, FRP1 ELISA Kit, FrzA ELISA Kit, SARP2 ELISA Kit, 2210415K03Rik ELISA Kit, AW011917 ELISA Kit, AW107218 ELISA Kit, AW742929 ELISA Kit, sFRP-1 ELISA Kit, CSFRP1 ELISA Kit, SFRP1 ELISA Kit, XfrzA ELISA Kit, frp ELISA Kit, frp-1 ELISA Kit, frp1 ELISA Kit, frzA ELISA Kit, sarp2 ELISA Kit, sfrp1 ELISA Kit, LOC100231868 ELISA Kit, Srfp1a ELISA Kit, wu:fd19b09 ELISA Kit, secreted frizzled related protein 1 ELISA Kit, secreted frizzled-related protein 1 ELISA Kit, Secreted frizzled-related protein 1 ELISA Kit, secreted frizzled-related protein 1a ELISA Kit, secreted frizzled-related protein 1b ELISA Kit, SFRP1 ELISA Kit, Sfrp1 ELISA Kit, sfrp1 ELISA Kit, sfrp1a ELISA Kit, sfrp1b ELISA Kit
- 基因ID
- 6422
- UniProt
- Q8N474
- 途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
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