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KPNA2 ELISA 试剂盒
KPNA2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
KPNA2ELISA Kit详情
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KPNA2
(Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
适用
All reactivities for KPNA2 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human Importin alpha 2/KPNA2/SRP1-alpha ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes .
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
KPNA2目标详情
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KPNA2
(Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
别名
Importin alpha 2 (KPNA2 产品 )
别名
IPOA1 ELISA Kit, QIP2 ELISA Kit, RCH1 ELISA Kit, SRP1alpha ELISA Kit, ima2 ELISA Kit, importin ELISA Kit, 2410044B12Rik ELISA Kit, PTAC58 ELISA Kit, Rch1 ELISA Kit, ipoa1 ELISA Kit, kpna2 ELISA Kit, qip2 ELISA Kit, rch1 ELISA Kit, srp1alpha ELISA Kit, hm:zeh0389 ELISA Kit, hm:zeh0389r ELISA Kit, zgc:113902 ELISA Kit, zgc:86945 ELISA Kit, karyopherin subunit alpha 2 ELISA Kit, importin subunit alpha-2 ELISA Kit, Importin subunit alpha-2 ELISA Kit, karyopherin (importin) alpha 2 ELISA Kit, karyopherin alpha-2 subunit like L homeolog ELISA Kit, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) ELISA Kit, KPNA2 ELISA Kit, EDI_246290 ELISA Kit, EDI_335040 ELISA Kit, ima2 ELISA Kit, Kpna2 ELISA Kit, kpna2.L ELISA Kit, kpna2 ELISA Kit
基因ID
3838
UniProt
P52292
途径
M Phase , Protein targeting to Nucleus
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