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MAP2K3 ELISA 试剂盒

MAP2K3 适用: 人, 小鼠, 大鼠 pSer189 Colorimetric Cell ELISA Cell Culture Cells
产品编号 ABIN4881290
发货至: 中国
  • 抗原 See all MAP2K3 ELISA试剂盒
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    抗原表位
    pSer189
    适用
    人, 小鼠, 大鼠
    检测方法
    Colorimetric
    实验类型
    Cell ELISA
    应用范围
    ELISA
    原理
    Cell-Based Human/Mouse/Rat MKK3 (S189) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    样品类型
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    特异性
    The antibodies provided in this kit recognizes human/mouse/rat MKK3 phosphorylated at Serine-189 and total MKK3 for comparison.
    产品特性
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    组件
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • 样本量
    100 μL
    板类型
    Uncoated
    实验流程
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all MAP2K3 ELISA试剂盒
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    别名
    MKK3 (MAP2K3 产品)
    别名
    MAPKK3 ELISA Kit, MEK3 ELISA Kit, MKK3 ELISA Kit, PRKMK3 ELISA Kit, SAPKK-2 ELISA Kit, SAPKK2 ELISA Kit, AW212142 ELISA Kit, Prkmk3 ELISA Kit, mMKK3b ELISA Kit, Mkk3 ELISA Kit, mitogen-activated protein kinase kinase 3 ELISA Kit, mitogen activated protein kinase kinase 3 ELISA Kit, MAP kinase activator XMEK3 ELISA Kit, MAP2K3 ELISA Kit, Map2k3 ELISA Kit, map2k3 ELISA Kit
    基因ID
    5606
    UniProt
    P46734
    途径
    MAPK Pathway, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
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