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MAP2K3 ELISA 试剂盒

MAP2K3 适用: 人, 小鼠, 大鼠 pSer189 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
产品编号 ABIN2748359
发货至: 中国
  • 抗原 See all MAP2K3 ELISA试剂盒
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    抗原表位
    pSer189, total
    适用
    人, 小鼠, 大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human/Mouse/Rat Phospho-MKK3 (S189) and Total MKK3 ELISA Kit. This assay semi-quantitatively measures phophorylated MKK3 (Ser189) and Total MKK3 in lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    The antibody pair provided in this kit recognizes human, mouse, and rat MKK3 phosphorylated at site Serine-189 and Total MKK3.
    产品特性
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MAP2K3 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all MAP2K3 ELISA试剂盒
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    别名
    MKK3 (MAP2K3 产品)
    别名
    MAPKK3 ELISA Kit, MEK3 ELISA Kit, MKK3 ELISA Kit, PRKMK3 ELISA Kit, SAPKK-2 ELISA Kit, SAPKK2 ELISA Kit, AW212142 ELISA Kit, Prkmk3 ELISA Kit, mMKK3b ELISA Kit, Mkk3 ELISA Kit, mitogen-activated protein kinase kinase 3 ELISA Kit, mitogen activated protein kinase kinase 3 ELISA Kit, MAP kinase activator XMEK3 ELISA Kit, MAP2K3 ELISA Kit, Map2k3 ELISA Kit, map2k3 ELISA Kit
    背景
    Mitogen-activated protein kinase kinase 3 (MKK3) phosphorylated at Serine-189 and Total MKK3
    基因ID
    5606
    UniProt
    P46734
    途径
    MAPK Pathway, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
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