MAP2K3 ELISA 试剂盒
This 人, 小鼠 和 大鼠 MAP2K3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 MAP2K3.
Quick Overview for MAP2K3 ELISA 试剂盒 (ABIN4881290)
抗原
See all MAP2K3 ELISA试剂盒
MAP2K3
(Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
抗原表位
pSer189
适用
人, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Culture Cells
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原理
- Cell-Based Human/Mouse/Rat MKK3 (S189) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibodies provided in this kit recognizes human/mouse/rat MKK3 phosphorylated at Serine-189 and total MKK3 for comparison.
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产品特性
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- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
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组件
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- uncoated 96-well Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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样本量
- 100 μL
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板类型
- Uncoated
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实验流程
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- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacture's instructions.
- Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
- Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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有效期
- 6 months
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- MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
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别名
- MKK3
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基因ID
- 5606
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UniProt
- P46734
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途径
- MAPK Pathway, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
抗原 See all MAP2K3 ELISA试剂盒
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