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TNFSF8 ELISA 试剂盒

TNFSF8 适用: 人 Soluble Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN456421
发货至: 中国
  • 抗原 See all TNFSF8 ELISA试剂盒
    TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
    抗原表位
    Soluble
    适用
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156-10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the specific measurement of human sCD30L concentrations in cell culture supernates, serum, and plasma.
    样品类型
    Cell Culture Supernatant, Serum, Plasma
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural human sCD30L.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    产品特性
    Homo sapiens,TNFSF8,Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,TNFSF8,CD30L,CD30LG,CD153,CD153
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sCD30L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD30L present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sCD30L is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD30L bound in the initial step. The color development is stopped and the intensity of the color is measured.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all TNFSF8 ELISA试剂盒
    TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
    别名
    TNFSF8 (TNFSF8 产品)
    别名
    CD153 ELISA Kit, CD30LG ELISA Kit, Cd30l ELISA Kit, CD30L ELISA Kit, TNFRSF8 ELISA Kit, TNFSF8 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 8 ELISA Kit, TNF superfamily member 8 ELISA Kit, tumor necrosis factor superfamily member 8 ELISA Kit, Tnfsf8 ELISA Kit, TNFSF8 ELISA Kit
    背景
    The human CD30 ligand, CD153, is a 40kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer Soluble trimeric CD153 binds to membrane-anchored CD30 with a relatively high affinity and is very effective in triggering cell death and TNFα. CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, as well as its involvement in disorders like a variety of lymphoid malignancies like Hodgkin’s disease and anaplastic large cell lymphoma.
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