TNFSF8 ELISA 试剂盒
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- 抗原 See all TNFSF8 ELISA试剂盒
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: CD153 antigen, CD30 antigen ligand, CD30 L, CD30 ligand, CD30L, CD30LG, MGC138144, TNFSF8, Tumor necrosis factor (ligand) superfamily member 8, Tumor necrosis factor ligand superfamily member 8
Background: The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
Gene Name: TNFSF8
Production: Natural and recombinant mouse CD30L. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: Q68-D239
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Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA Kit
TNFSF8 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 小鼠 AA 68-239 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CD30L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CD30L standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 15.6pg/mL-1000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all TNFSF8 ELISA试剂盒
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- 别名
- CD30L (TNFSF8 产品)
- 别名
- CD153 ELISA Kit, CD30LG ELISA Kit, Cd30l ELISA Kit, CD30L ELISA Kit, TNFRSF8 ELISA Kit, TNFSF8 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 8 ELISA Kit, TNF superfamily member 8 ELISA Kit, tumor necrosis factor superfamily member 8 ELISA Kit, Tnfsf8 ELISA Kit, TNFSF8 ELISA Kit
- 基因ID
- 21949
- NCBI登录号
- NP_033429
- UniProt
- P32972
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