IL-6 ELISA 试剂盒
(2 references)-
- 抗原 See all IL-6 (IL6) ELISA试剂盒
- IL-6 (IL6) (Interleukin 6 (IL6))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse IL-6 concentrations in cell culture supernates, serum, plasma and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural mouse IL-6.
- 交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
- 灵敏度
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
- 产品特性
- Mus musculus,Mouse,Interleukin-6,IL-6,B-cell hybridoma growth factor,Interleukin HP-1,Il6,Il-6
- Featured
- Discover our best selling IL6 ELISA Kit
- Top Product
- Discover our top product IL6 ELISA Kit
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Interleukin 6 (IL6) ELISA Kit
IL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 6 (IL6) ELISA KitIL6 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 AA 29-212 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 小鼠 AA 25-211 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 大鼠 AA 25-211 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 兔 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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: "Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways." in: International journal of molecular medicine, Vol. 31, Issue 6, pp. 1484-94, (2013) (PubMed).
: "Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages." in: The Journal of pharmacology and experimental therapeutics, Vol. 336, Issue 2, pp. 381-90, (2011) (PubMed).
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: "Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways." in: International journal of molecular medicine, Vol. 31, Issue 6, pp. 1484-94, (2013) (PubMed).
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- 抗原 See all IL-6 (IL6) ELISA试剂盒
- IL-6 (IL6) (Interleukin 6 (IL6))
- 别名
- Il6 (IL6 产品)
- 别名
- BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, IL-6 ELISA Kit, Il-6 ELISA Kit, ILg6 ELISA Kit, Ifnb2 ELISA Kit, il6 ELISA Kit, CHIL-6 ELISA Kit, interleukin 6 ELISA Kit, interleukin-6 ELISA Kit, IL6 ELISA Kit, Il6 ELISA Kit, il-6 ELISA Kit, IL-6 ELISA Kit
- 背景
- Interleukin 6, or IL-6, is a 21 kDa multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response, and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1 β and TNFa act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6 specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3 causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NF-IL-6 transcription. Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses.
- 途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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