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IL-6 ELISA 试剂盒

Name: IL-6 ELISA 试剂盒
SKU: ABIN921064
Availability: OutOfStock

大鼠 IL-6 ELISA Kit, Colorimetric is an assay for quantification of 大鼠 IL-6, that has been mentioned in 144+ publications.

ELISA

2 images

(144 references)

产品编号 ABIN921064

发货至: 中国

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Quick Overview for IL-6 ELISA 试剂盒 (ABIN921064)

抗原

See all IL-6 (IL6) ELISA试剂盒

IL-6 (IL6) (Interleukin 6 (IL6))

抗原表位

AA 25-211

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product IL6 ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich Rat IL-6/Interleukin-6 ELISA Kit to quantitate Rat Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: F25-T211
Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 5 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 6 (IL6) ELISA Kit
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IL6 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 6 (IL6) ELISA Kit
IL6 适用: 小鼠 AA 25-211 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Interleukin 6 (IL6) ELISA Kit
IL6 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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IL6 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 6 (IL6) ELISA Kit
IL6 适用: 人 AA 29-212 Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Interleukin 6 (IL6) ELISA Kit
IL6 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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IL6 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 6 (IL6) ELISA Kit
IL6 适用: 兔 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 5%, 6.2%, 5.9%
Inter-Assay Precision (CV%): 5.5%, 7.1%, 7.5%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
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Piao, Liu, Guo, Meng, Gao: "Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 7085709, (2018) (PubMed).

Chen, Wu, Chen, Xie, Fang, Hu, Chen, Fu, He: "Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 143, (2018) (PubMed).

Xiong, Ru, Chen, Tian, Li: "Mitochondrial dysfunction and inflammatory response in the cytotoxicity of NR8383 macrophages induced by fine particulate matter." in: Environmental toxicology and pharmacology, Vol. 55, pp. 1-7, (2018) (PubMed).

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Tagliari, Campos, Costa-Casagrande, Salvalaggio: "THE IMPACT OF THE USE OF SYMBIOTICS IN THE PROGRESSION OF NONALCOHOLIC FATTY LIVER DISEASE IN A RAT MODEL." in: Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery, Vol. 30, Issue 3, pp. 211-215, (2018) (PubMed).

Gao, Meng, Cui: "Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat." in: Experimental lung research, Vol. 43, Issue 9-10, pp. 439-446, (2018) (PubMed).

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Ling, Feng, Wei, Wang, Wang, Zhang, He, Wang, Zeng, Xiong: "Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 283, (2018) (PubMed).

Zhang, Xu, Hu, Wu, Qin, Zhang: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).

Vidal, Cascão, Finnilä, Lopes, Saarakkala, Zioupos, Canhão, Fonseca: "Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis." in: PLoS ONE, Vol. 13, Issue 1, pp. e0190920, (2018) (PubMed).

You, Zu, Liu, Kong, Song, Wei, Zhou, Wang, Yan: "Synovial fibroblast-targeting liposomes encapsulating an NF-κB-blocking peptide ameliorates zymosan-induced synovial inflammation." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2449-2457, (2018) (PubMed).

Gao, Jing, Zhang, Wu et al.: "Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor ..." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1029-1035, (2018) (PubMed).

Xu, Qin, Hu, Mu, Zhu, Lu, Luo: "Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 83, (2018) (PubMed).

Chen, Chen, Fan, Wu, Yang, Fang, Fu, Li et al.: "Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental ..." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 116, (2018) (PubMed).

Chen, Ye, Chen, Shi, Wu, Lin, Lin, Li, Fu, Li: "Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 150, (2018) (PubMed).

Zhao, Han: "Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy." in: Medical science monitor basic research, Vol. 24, pp. 84-92, (2018) (PubMed).

Zou, Huang, Lu, Fang, Wang, Zhao, Jia, Xu, Wang, Wang, Dong: "The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 165, (2017) (PubMed).

Chen, Huang, Tu, Lu, Xie, Zhao, Wu, Shi, Fang, Wei, Zhang, Lu: "NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation." in: Neuroscience, Vol. 356, pp. 193-206, (2017) (PubMed).

Ye, Shen, Huang, Zhang, Xiao: "Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model." in: Journal of the science of food and agriculture, Vol. 97, Issue 13, pp. 4548-4556, (2017) (PubMed).

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抗原 See all IL-6 (IL6) ELISA试剂盒

IL-6 (IL6) (Interleukin 6 (IL6))

别名

IL6

背景

Background: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.
Gene Full Name: interleukin 6

基因ID

24498

UniProt

P20607

途径

TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome