STAT3 ELISA 试剂盒
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- 抗原 See all STAT3 ELISA试剂盒
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- TransAM STAT3 Kits are tested for sensitivity in detecting STAT activation.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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- Discover our best selling STAT3 ELISA Kit
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- Discover our top product STAT3 ELISA Kit
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Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA Kit
STAT3 适用: 人, 大鼠, 小鼠 pTyr705 Colorimetric Cell ELISA Cell Culture Cells
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 人, 大鼠, 小鼠 pTyr705 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 人, 大鼠, 小鼠 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) ELISA KitSTAT3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Nuclear extracts prepared from HepG2 cells that are either unstimulated or stimulated with IL-6 are diluted to 0.625 μg/well and assayed using the TransAM STAT3 Kit. The ratio of the signals from the stimulated over unstimulated cells must be above 4. Lot No.04114017 was developed for 10 minutes. It gave a ratio of 4.8 at 5ug/well (Figure 1). This ratio may vary depending on the basal level of STAT activation in a given cell type and the stimulation used. TransAM STAT3 Kits are also tested for specificity in detecting STAT activation. TransAM STAT assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated STAT consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents STAT binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on STAT binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the antibody, oligonucleotides, DTT, and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all STAT3 ELISA试剂盒
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
- 别名
- Stat3 (STAT3 产品)
- 别名
- 1110034C02Rik ELISA Kit, AW109958 ELISA Kit, Aprf ELISA Kit, APRF ELISA Kit, HIES ELISA Kit, Xstat3 ELISA Kit, aprf ELISA Kit, hies ELISA Kit, stat3 ELISA Kit, wu:fc15d02 ELISA Kit, wu:fl59g06 ELISA Kit, z-Stat3 ELISA Kit, signal transducer and activator of transcription 3 ELISA Kit, signal transduction and activation of transcription 3 ELISA Kit, signal transducer and activator of transcription 3, gene 1 L homeolog ELISA Kit, signal transducer and activator of transcription 3 (acute-phase response factor) ELISA Kit, STAT3 ELISA Kit, stat3 ELISA Kit, Stat3 ELISA Kit, stat3.1.L ELISA Kit
- 途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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