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p53 ELISA 试剂盒

Name: p53 ELISA 试剂盒
SKU: ABIN2748488
Availability: OutOfStock

This 人, 大鼠和小鼠 p53 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 大鼠和小鼠 p53. This ELISA Kit has been cited in1 publication.

(1 reference)

产品编号 ABIN2748488

发货至: 中国

Quick Overview for p53 ELISA 试剂盒 (ABIN2748488)

抗原

See all p53 (TP53) ELISA试剂盒

p53 (TP53) (Tumor Protein P53 (TP53))

抗原表位

pSer15

适用

人, 大鼠, 小鼠

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Culture Cells

Discover our top product TP53 ELISA Kit

原理

Cell-Based Human/Rat/Mouse p53 (S15) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Analytical Method

Semi-Quantitative

特异性

The antibodies provided in this kit recognizes Human/Rat/Mouse p53 phosphorylated at Serine-15 and total p53 for comparison.

产品特性
- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
组件
- uncoated 96-well Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 acetylated Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 AA 1-393 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Lysate

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract

Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate

样本量

100 μL

板类型

Uncoated

实验流程
1. Seed 10,000-30,000 cells into each well and incubate overnight.
2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
实验流程

Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

有效期

6 months
Hassan, Khan, Aman, Qamar, Ebaid, Al-Tamimi, Alhazza, Rady: "Restrained management of copper level enhances the antineoplastic activity of imatinib in vitro and in vivo." in: Scientific reports, Vol. 8, Issue 1, pp. 1682, (2018) (PubMed).
抗原 See all p53 (TP53) ELISA试剂盒

p53 (TP53) (Tumor Protein P53 (TP53))

别名

P53

背景

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

基因ID

7157

UniProt

P04637

途径

p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect