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MMP2 ELISA 试剂盒

MMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.614-150 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2748371
发货至: 中国
  • 抗原 See all MMP2 ELISA试剂盒
    MMP2 (Matrix Metalloproteinase 2 (MMP2))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.614-150 ng/mL
    最低检测浓度
    0.614 ng/mL
    应用范围
    ELISA
    原理
    Rat MMP-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Rat MMP-2.
    灵敏度
    0.6 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples3 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all MMP2 ELISA试剂盒
    MMP2 (Matrix Metalloproteinase 2 (MMP2))
    别名
    MMP-2 (MMP2 产品)
    别名
    2-MMP ELISA Kit, CG1794 ELISA Kit, DM2-MMP ELISA Kit, Dm2-MMP ELISA Kit, Dmel\\CG1794 ELISA Kit, MMP2 ELISA Kit, anon-WO0118547.84 ELISA Kit, dm-2MMP ELISA Kit, dmmp2 ELISA Kit, l(2)02353 ELISA Kit, mmp2 ELISA Kit, wu:fa99h12 ELISA Kit, wu:fk89d01 ELISA Kit, fgmmp-2 ELISA Kit, Mmp-2 ELISA Kit, LOC100135793 ELISA Kit, Clg4a ELISA Kit, GelA ELISA Kit, MMP-2 ELISA Kit, CLG4 ELISA Kit, CLG4A ELISA Kit, MMP-II ELISA Kit, MONA ELISA Kit, TBE-1 ELISA Kit, matrix metallopeptidase 2 ELISA Kit, Matrix metalloproteinase 2 ELISA Kit, matrix metalloproteinase 2 ELISA Kit, matrix metalloproteinase-2 ELISA Kit, MMP2 ELISA Kit, Mmp2 ELISA Kit, mmp2 ELISA Kit, LOC657982 ELISA Kit, LOC100135793 ELISA Kit
    背景
    MMP-2
    基因ID
    81686
    UniProt
    P33436
    途径
    Activation of Innate immune Response
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