MMP2 ELISA 试剂盒
(19 references)-
- 抗原 See all MMP2 ELISA试剂盒
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- 抗原表位
- AA 30-662
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 625-40000 pg/mL
- 最低检测浓度
- 625 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-2
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A30-C662 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: A30-C662 - Featured
- Discover our best selling MMP2 ELISA Kit
- Top Product
- Discover our top product MMP2 ELISA Kit
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 马 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 AA 30-660 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 大鼠 AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
SensoLyte® 520 MMP-2 Assay KitFRET Fluorometric
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: Pig Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase M10A family.
- 板类型
- Pre-coated
- 实验流程
- mouse MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-2 has been precoated onto 96-well plates. Standards(NSO, A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 40000pg/mL, 20,000pg/mL,10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL mouse MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MMP-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1428, Standard deviation: 75.7, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 3727, Standard deviation: 178.9, CV(%): 4.8
- Sample 3: n=16, Mean(pg/ml): 6658, Standard deviation: 412.8, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 1634, Standard deviation: 104.6, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 3967, Standard deviation: 218.2, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 6832, Standard deviation: 499, CV(%): 7.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b." in: Oncogene, Vol. 36, Issue 33, pp. 4692-4705, (2017) (PubMed).
: "Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-κB and JAK2-STAT3 signaling pathways." in: Journal of ethnopharmacology, Vol. 207, pp. 174-183, (2017) (PubMed).
: "Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma." in: Oncology letters, Vol. 13, Issue 5, pp. 2897-2902, (2017) (PubMed).
: "Role of sphingosine kinase/S1P axis in ECM remodeling of cardiac cells elicited by relaxin." in: Molecular endocrinology (Baltimore, Md.), Vol. 29, Issue 1, pp. 53-67, (2016) (PubMed).
: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).
: "MMP-9-Dependent Serum-Borne Bioactivity Caused by Multiwalled Carbon Nanotube Exposure Induces Vascular Dysfunction via the CD36 Scavenger Receptor." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 150, Issue 2, pp. 488-98, (2016) (PubMed).
: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).
: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." in: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).
: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).
: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." in: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).
: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." in: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).
: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." in: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).
: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).
: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." in: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).
: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).
: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).
: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).
: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).
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: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." in: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, (2018) (PubMed).
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- 抗原 See all MMP2 ELISA试剂盒
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- 别名
- MMP2 (MMP2 产品)
- 别名
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- 背景
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Protein Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity). .
Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.
Synonyms: 72 kDa type IV collagenase,3.4.24.24,72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2,PEX,Mmp2,
Full Gene Name: 72 kDa type IV collagenase
Cellular Localisation: Isoform 1: Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).. - 基因ID
- 17390
- UniProt
- P33434
- 途径
- Activation of Innate immune Response
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