Midkine ELISA 试剂盒
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- 抗原 See all Midkine (MDK) ELISA试剂盒
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.12-30 ng/mL
- 最低检测浓度
- 0.12 ng/mL
- 应用范围
- ELISA
- 原理
- Human Midkine ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1
- 灵敏度
- 0.12 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kit
MDK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 AA 23-143 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Midkine (MDK) ELISA试剂盒
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
- 别名
- Midkine (MDK 产品)
- 别名
- ARAP ELISA Kit, MK ELISA Kit, NEGF2 ELISA Kit, Mek ELISA Kit, MK-B ELISA Kit, PTF-alpha-1 ELISA Kit, mdk ELISA Kit, midkine ELISA Kit, negf2 ELISA Kit, RIHB ELISA Kit, cb518 ELISA Kit, fb79b05 ELISA Kit, fj33b02 ELISA Kit, fj48a12 ELISA Kit, mdk2 ELISA Kit, plei2 ELISA Kit, wu:fb79b05 ELISA Kit, wu:fj33b02 ELISA Kit, wu:fj48a12 ELISA Kit, MK-A ELISA Kit, PTF-alpha-2 ELISA Kit, X-PTF-alpha1 ELISA Kit, XMK ELISA Kit, mdk-a ELISA Kit, mdk-b ELISA Kit, midkine ELISA Kit, midkine L homeolog ELISA Kit, midkine (neurite growth-promoting factor 2) ELISA Kit, midkine b ELISA Kit, midkine S homeolog ELISA Kit, MDK ELISA Kit, Mdk ELISA Kit, mdk.L ELISA Kit, mdkb ELISA Kit, mdk.S ELISA Kit
- 背景
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Gene Names: MDK MK1 NEGF2
Protein names: Midkine (MK) (Amphiregulin-associated protein) (ARAP) (Midgestation and kidney protein) (Neurite outgrowth-promoting factor 2) (Neurite outgrowth-promoting protein)
- 基因ID
- 4192
- UniProt
- P21741
- 途径
- RTK signaling, M Phase, Skeletal Muscle Fiber Development
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