PD-L1 ELISA 试剂盒
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 46-3000 pg/mL
- 最低检测浓度
- 46 pg/mL
- 应用范围
- ELISA
- 原理
- Human B7-H1 (CD274) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human B7-H1 (CD274)
- 灵敏度
- 46 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PD-L1 ELISA Kit
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- Discover our top product PD-L1 ELISA Kit
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CD274 (PD-L1) ELISA Kit
PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
CD274 (PD-L1) ELISA KitWide Range PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PD-L1 Inhibitor Screening ELISA KitELISA, ScA 适用: 人 Colorimetric
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 AA 19-239 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 AA 19-238 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
- 别名
- B7H1/PD-L1 (PD-L1 产品)
- 别名
- B7-H ELISA Kit, B7H1 ELISA Kit, PD-L1 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PDL1 ELISA Kit, A530045L16Rik ELISA Kit, B7h1 ELISA Kit, Pdcd1l1 ELISA Kit, Pdcd1lg1 ELISA Kit, Pdl1 ELISA Kit, RGD1566211 ELISA Kit, CD274 molecule ELISA Kit, CD274 antigen ELISA Kit, programmed cell death 1 ligand 2 ELISA Kit, CD274 ELISA Kit, Cd274 ELISA Kit, PDCD1LG2 ELISA Kit
- 背景
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Gene Names: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1
Protein names: Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)
- 基因ID
- 29126
- UniProt
- Q9NZQ7
- 途径
- Cancer Immune Checkpoints
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