IL1A ELISA 试剂盒
Quick Overview for IL1A ELISA 试剂盒 (ABIN2648756)
抗原
See all IL1A ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 1 pg/ml
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产品特性
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 山羊 Colorimetric Sandwich ELISA 0.781 pg/mL - 50 pg/mL Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 大鼠 AA 115-270 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鸡 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody-IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1α standard dilutions and IL-1α sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL-1a
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原 See all IL1A ELISA试剂盒
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