IL-3 ELISA 试剂盒
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- 抗原 See all IL-3 ELISA试剂盒
- IL-3 (Interleukin 3 (IL-3))
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适用
- 人
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.
- 样品类型
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- 灵敏度
- 15 pg/ml
- 产品特性
- Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Discover our top product IL-3 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 3.5 h
- 板类型
- Pre-coated
- 实验流程
- Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IL-3 ELISA试剂盒
- IL-3 (Interleukin 3 (IL-3))
- 别名
- IL-3 (IL-3 产品)
- 别名
- BPA ELISA Kit, Csfmu ELISA Kit, HCGF ELISA Kit, Il-3 ELISA Kit, MCGF ELISA Kit, PSF ELISA Kit, IL-3 ELISA Kit, MULTI-CSF ELISA Kit, interleukin 3 ELISA Kit, interleukin 3 (colony-stimulating factor, multiple) ELISA Kit, Il3 ELISA Kit, IL3 ELISA Kit
- 途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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