WNT5B ELISA 试剂盒
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- 抗原 See all WNT5B ELISA试剂盒
- WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56-100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.53 ng/mL
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Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit
WNT5B 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA KitWNT5B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA KitWNT5B 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
- Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all WNT5B ELISA试剂盒
- WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))
- 别名
- Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) (WNT5B 产品)
- 别名
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- 途径
- WNT signaling, Embryonic Body Morphogenesis, Positive Regulation of fat Cell Differentiation
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