IL-6 ELISA 试剂盒
Quick Overview for IL-6 ELISA 试剂盒 (ABIN2043815)
抗原
See all IL-6 (IL6) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 3.12 pg/mL
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灵敏度
- < 1.29 pg/mL
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板类型
- Pre-coated
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实验流程
- Interleukin 6 (IL6) BioAssay-ELISA Kit (Sheep) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL-6 (IL6) (Interleukin 6 (IL6))
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途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-6 (IL6) ELISA试剂盒
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