HVEM ELISA 试剂盒
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- 抗原 See all HVEM (TNFRSF14) ELISA试剂盒
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- 抗原表位
- AA 29-207
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF14/HVEM
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: Q29-V207 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: Q29-V207 - Featured
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Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) ELISA Kit
TNFRSF14 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) ELISA KitTNFRSF14 适用: 人 AA 39-202 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse TNFRSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF14 has been precoated onto 96-well plates. Standards(CHO, Q29-V207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF14 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFRSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF14 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 58, Standard deviation: 2.5, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 260, Standard deviation: 9.36, CV(%): 3.6
- Sample 3: n=16, Mean(pg/ml): 594, Standard deviation: 30.3, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 64, Standard deviation: 3.58, CV(%): 5.6
- Sample 2: n=24, Mean(pg/ml): 286, Standard deviation: 12.87, CV(%): 4.5
- Sample 3: n=24, Mean(pg/ml): 654, Standard deviation: 39.24, CV(%): 6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all HVEM (TNFRSF14) ELISA试剂盒
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- 别名
- TNFRSF14 (TNFRSF14 产品)
- 别名
- ATAR ELISA Kit, CD270 ELISA Kit, HVEA ELISA Kit, HVEM ELISA Kit, LIGHTR ELISA Kit, TR2 ELISA Kit, Atar ELISA Kit, HveA ELISA Kit, Hvem ELISA Kit, Tnfrs14 ELISA Kit, TNF receptor superfamily member 14 ELISA Kit, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) ELISA Kit, Tnfrsf14 ELISA Kit, TNFRSF14 ELISA Kit
- 物质类
- Viral Protein
- 背景
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Background: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Synonyms: Protein Tnfrsf14 ,Tumor necrosis factor receptor superfamily member 14 ,Tnfrsf14 ,
Full Gene Name: tumor necrosis factor receptor superfamily, member 14
- 基因ID
- 230979
- UniProt
- Q80WM9
- 途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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