TWEAK ELISA 试剂盒
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- 抗原 See all TWEAK (TNFSF12) ELISA试剂盒
- TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
- 抗原表位
- AA 94-249
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF12
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S94-H249 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S94-H249 - Featured
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- Discover our top product TNFSF12 ELISA Kit
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Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kit
TNFSF12 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 小鼠 AA 94-249 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 大鼠 AA 94-249 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA KitTNFSF12 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the tumor necrosis factor family.
Tissue Specificity: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
- 板类型
- Pre-coated
- 实验流程
- human TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF12 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFSF12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFSF12 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 588, Standard deviation: 26.46, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 1202, Standard deviation: 65, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 2235, Standard deviation: 84.93, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 634, Standard deviation: 37.41, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 1127, Standard deviation: 72.13, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 2446, Standard deviation: 115, CV(%): 4.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all TWEAK (TNFSF12) ELISA试剂盒
- TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
- 别名
- TNFSF12 (TNFSF12 产品)
- 别名
- APO3L ELISA Kit, DR3LG ELISA Kit, TWEAK ELISA Kit, Apo3l ELISA Kit, Dr3l ELISA Kit, Dr3lg ELISA Kit, Tweak ELISA Kit, TNF superfamily member 12 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 12 ELISA Kit, TNFSF12 ELISA Kit, Tnfsf12 ELISA Kit
- 背景
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Protein Function: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. .
Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis(TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
Synonyms: Tumor necrosis factor ligand superfamily member 12,APO3 ligand,TNF-related weak inducer of apoptosis,TWEAK,Tumor necrosis factor ligand superfamily member 12, membrane form,Tumor necrosis factor ligand superfamily member 12, secreted form,TNFSF12,APO3L, DR3LG,UNQ181/PRO207,
Full Gene Name: Tumor necrosis factor ligand superfamily member 12
Cellular Localisation: Cell membrane, Single-pass type II membrane protein. - 基因ID
- 8742
- UniProt
- O43508
- 途径
- Apoptosis
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