TRKB ELISA 试剂盒
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- 抗原 See all TRKB (NTRK2) ELISA试剂盒
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.8-6000 pg/mL
- 最低检测浓度
- 93.8 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of mouse TrkB in tissue lysates or cell culture supernates.
- 样品类型
- Tissue Lysate, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 灵敏度
- < 5 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Mouse trkB antibody 2. Lyophilized Mouse trkB standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse trkB antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
- Featured
- Discover our best selling NTRK2 ELISA Kit
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- Discover our top product NTRK2 ELISA Kit
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Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kit
NTRK2 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 AA 32-429 Colorimetric Sandwich ELISA 93.8-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 AA 32-430 Colorimetric Sandwich ELISA
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TrkB polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TrkB polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the TrkB amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of TrkB can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all TRKB (NTRK2) ELISA试剂盒
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
- 别名
- TRKB (NTRK2 产品)
- 别名
- trkb ELISA Kit, trkb-b ELISA Kit, xtrkb ELISA Kit, si:ch211-220b11.1 ELISA Kit, trkB2 ELISA Kit, NTRK2 ELISA Kit, ntrk2 ELISA Kit, gp145-trkb ELISA Kit, TRKB ELISA Kit, GP145-TrkB ELISA Kit, trk-B ELISA Kit, AI848316 ELISA Kit, C030027L06Rik ELISA Kit, Tkrb ELISA Kit, trkB ELISA Kit, RATTRKB1 ELISA Kit, TRKB1 ELISA Kit, Trk-B ELISA Kit, TrkB ELISA Kit, neurotrophic receptor tyrosine kinase 2 ELISA Kit, neurotrophic receptor tyrosine kinase 2 L homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2b ELISA Kit, neurotrophic receptor tyrosine kinase 2 S homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2 ELISA Kit, NTRK2 ELISA Kit, ntrk2.L ELISA Kit, ntrk2b ELISA Kit, ntrk2.S ELISA Kit, ntrk2 ELISA Kit, Ntrk2 ELISA Kit
- 背景
- TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene. The TrkB receptor is part of the large family of receptor tyrosine kinases, it is located at the cellular membrane, and is activated by binding of a ligand to the receptor's extracellular domain. TrkB is the high affinity catalytic receptor for several neurotrophins, which are small protein growth factors that induce the survival and differentiation of distinct cell populations.
- 途径
- RTK signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
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