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|抗原||RAD23 Homolog A (S. Cerevisiae) (RAD23A) 抗体|
|标记||This RAD23A antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
RAD23A抗体详情RAD23A目标详情 使用细节 贮存及处理 References for anti-RAD23A antibody (ABIN357606) 图像
|特异性||This antibody is specific to RAD23A (C-term). Predicted to cross react with Mouse (100 % Antigen Homology).|
|纯化方法||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|免疫原||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RAD23A.|
|Plasmids, Primers & others|
RAD23A目标详情RAD23A抗体详情 使用细节 贮存及处理 References for anti-RAD23A antibody (ABIN357606) 图像 回页首
|别名||RAD23A (RAD23A Antibody 摘要)|
|背景||RAD223A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23A, UV excision repair protein RAD23 homolog A|
|途径||DNA Damage Repair|
使用细节RAD23A抗体详情 RAD23A目标详情 贮存及处理 References for anti-RAD23A antibody (ABIN357606) 图像 回页首
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理RAD23A抗体详情 RAD23A目标详情 使用细节 References for anti-RAD23A antibody (ABIN357606) 图像 回页首
|缓冲液||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-RAD23A antibody (ABIN357606)RAD23A抗体详情 RAD23A目标详情 使用细节 贮存及处理 图像 回页首
Mueller, Feigon: "Structural determinants for the binding of ubiquitin-like domains to the proteasome." in: The EMBO journal, Vol. 22, Issue 18, pp. 4634-45, 2003
Elder, Song, Chen, Hopkins, Lieberman, Zhao: "Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination." in: Nucleic acids research, Vol. 30, Issue 2, pp. 581-91, 2002
Mueller, Feigon: "Solution structures of UBA domains reveal a conserved hydrophobic surface for protein-protein interactions." in: Journal of molecular biology, Vol. 319, Issue 5, pp. 1243-55, 2002
Chen, Shinde, Ortolan, Madura: "Ubiquitin-associated (UBA) domains in Rad23 bind ubiquitin and promote inhibition of multi-ubiquitin chain assembly." in: EMBO reports, Vol. 2, Issue 10, pp. 933-8, 2001
Gaynor, Chen: "Analysis of apoptosis induced by HIV-1 Vpr and examination of the possible role of the hHR23A protein." in: Experimental cell research, Vol. 267, Issue 2, pp. 243-57, 2001
图像RAD23A抗体详情 RAD23A目标详情 使用细节 贮存及处理 References for anti-RAD23A antibody (ABIN357606) 回页首