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|抗原||Endonuclease G (ENDOG) 抗体|
|适用||人, 小鼠, 大鼠 其他选择|
|标记||This Endonuclease G antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Endonuclease G抗体详情Endonuclease G目标详情 使用细节 贮存及处理 图像
|特异性||EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.|
|纯化方法||Affinity chromatography purified via peptide column|
|免疫原||Human EndoG Peptide|
|Plasmids, Primers & others|
Endonuclease G目标详情Endonuclease G抗体详情 使用细节 贮存及处理 图像 回页首
|别名||Endonuclease G (ENDOG Antibody 摘要)|
|背景||The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).Synonyms: ENDOG|
使用细节Endonuclease G抗体详情 Endonuclease G目标详情 贮存及处理 图像 回页首
ELISA. Western 1 to 2 μg/mL. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理Endonuclease G抗体详情 Endonuclease G目标详情 使用细节 图像 回页首
|缓冲液||PBS containing 0.02 % sodium azide.|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存方法||Store the antibody undiluted at 2-8 °C.|