We are preparing the requested document. Please wait, this may take a while...!
There was a problem generating the requested document, please try again.
|抗原||Endonuclease G (ENDOG) 抗体|
|抗原表位||AA 200-250 其他选择|
|适用||大鼠, 小鼠, 人 其他选择|
|标记||This Endonuclease G antibody is un-conjugated 其他选择|
Flow Cytometry (FACS), Western Blotting (WB)
Endonuclease G抗体详情Endonuclease G目标详情 使用细节 贮存及处理 图像
|特异性||EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney|
|交叉反应 （详细）||EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney|
|免疫原||KLH-coupled synthetic peptide within AA 200–250 of human EndoG|
|Plasmids, Primers & others|
Endonuclease G目标详情Endonuclease G抗体详情 使用细节 贮存及处理 图像 回页首
|别名||EndoG (ENDOG Antibody 摘要)|
|背景||Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis.Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200-50 of human EndoG (Swiss Prot: Q14249).|
使用细节Endonuclease G抗体详情 Endonuclease G目标详情 贮存及处理 图像 回页首
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
贮存及处理Endonuclease G抗体详情 Endonuclease G目标详情 使用细节 图像 回页首
|缓冲液||PBS, pH 7.4, containing 0.02 % sodium azide|
|注意事项||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|储存条件||4 °C/-20 °C|
|储存方法||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
图像Endonuclease G抗体详情 Endonuclease G目标详情 使用细节 贮存及处理 回页首