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Polyclonal Antibodies

多克隆抗体（pAbs）是由多个B细胞克隆产生的免疫球蛋白的异质性混合物，能够识别同一抗原上的多个表位。这种广泛的表位识别赋予其高灵敏度和稳健的信号生成能力，使其特别适用于检测低丰度蛋白或具有结构变异的靶标。

由于能够结合抗原的不同区域，多克隆抗体在表位部分变性或被遮蔽的情况下仍能保持良好性能，例如在Western blot或免疫组织化学（IHC）中。它们广泛应用于ELISA、WB、IHC、IF和免疫沉淀等实验中，适用于需要强且稳定信号检测的场景。

与此同时，其来源于动物的生产方式会导致批次间差异，从而影响长期重复性。此外，多表位结合可能导致其特异性低于单克隆或重组抗体。因此，多克隆抗体更适用于探索性研究、低表达靶标检测，或对灵敏度要求高于特异性的实验。

对于需要高度批次一致性或精确靶标区分的应用，单克隆或重组抗体通常更为合适。多克隆混合抗体（multiclonal antibodies）则通过结合广泛表位识别和更好的重复性提供了一种平衡方案。

在antibodies-online，我们的多克隆抗体产品组合包含用于ELISA、Western blot、免疫组织化学、免疫荧光和免疫沉淀的验证抗体。凭借广泛的靶标覆盖和应用验证数据，这些抗体可为多种研究应用提供可靠的蛋白检测工具。

重要靶标

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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588,520 results

Fibronectin 抗体 (AA 1201-1300)

适用: 人, 大鼠, 小鼠, Cow WB, ICC, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

Image kindly submitted by Edita Aksamitiene as part of the free sample program.

Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (ABIN671646) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Image provided by One World Lab validation program.

Paraformaldehyde-fixed, paraffin embedded rat intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Downregulation of miR-27a with a miR-27a inhibitor prevented high glucose (HG)-induced mesangial cell (MC) proliferation and the expression of ECM-associated profibrotic genes via upregulation of PPARγ expression.

Inhibition of miR-27a with antagomir-27a reduces the expression of profibrotic genes and ECM proteins in the kidney glomeruli of STZ-induced diabetic rats.

7 images

13 references

产品编号 ABIN671646

100 μL

发货至: 中国

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Collagen Type I 抗体 (AA 1321-1400)

COL1 适用: 人, 小鼠, 大鼠, 兔, 鱼, 犬 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated

SW480 lysates probed with Rabbit Anti-Pro-Collagen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:300 followed by conjugation to the secondary PE antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse muscle lysates probed with Rabbit Anti-Pro-Collegen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

16 references

产品编号 ABIN670386

100 μL

发货至: 中国

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CD31 抗体 (AA 601-680)

PECAM1 适用: 人, 小鼠, 犬 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

Recombinant human CD31 protein probed with CD31 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed: Endothelial cells labeled with Anti-CD31/PECAM-1 Polyclonal Antibody, Unconjugated (ABIN669006) at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated at 1:200 for 40 minutes at 37°

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Rat spleen cells probed with Rabbit Anti-CD31 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

8 images

16 references

产品编号 ABIN669006

100 μL

发货至: 中国

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HNE 抗体

HNE WB, ELISA, IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

The diabetes effect on IR affected cardiac lipid peroxidation in MDA content (A, n = 6), 4-HNE expression (B, n = 3), leukocyte infiltration and cardiac structure (C,D, n = 5), microcirculation (E,F, n = 10) and infarct size (G,H, n = 5) in IR CON and IR STZ rats.

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (ABIN873270) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Antigen: 0.

Mesenteric vascular Western blot graph (A) and analysis of 4-HNE (B), CaSR (C), p-eNOS (D) and BAG3 (E) are demonstrated.

6 images

13 references

产品编号 ABIN873270

100 μL

发货至: 中国

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SNAP Tag 抗体

ELISA, SEM, WB 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-SNAP Tag antibody (ABIN1573927)

Immunofluorescence validation image for anti-SNAP Tag antibody (ABIN1573927)

Electron Microscopy validation image for anti-SNAP Tag antibody (ABIN1573927)

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD) Lane 3: Negative 293 cell lysate Primary antibody: 1 µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (ABIN398494) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 10,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939)

4 images

3 references

Independent Validation

3 validations

产品编号 ABIN1573927

40 μg

发货至: 中国

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Independently Validated

ELF1 抗体 (C-Term)

Verified ELF1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

Western Blotting validation image for anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody (ABIN184790)

ABIN184790 (0.

ABIN184790 staining (0.

ABIN184790 (2.

ABIN184790 (0.

5 images

Independent Validation

1 validation

产品编号 ABIN184790

100 μg

发货至: 中国

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Independently Validated

TBX2/3 抗体

适用: 人, 小鼠, 大鼠 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-TBX2/3 antibody (ABIN6265491)

Cleavage Under Targets and Release Using Nuclease validation image for anti-TBX2/3 antibody (ABIN6265491)

Western blot analysis of TBX3 expression in mouse brain lysate

ABIN6266698 staining A549 by IF/ICC.

Western blot analysis on A549 cell lysate using TBX3 Antibody.

5 images

Independent Validation

1 validation

产品编号 ABIN6265491

100 μL (More quantities available)

发货至: 中国

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Independently Validated

Histone H4 抗体 (3meLys20)

适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Histone H4 (3meLys20) antibody (ABIN7260401)

Western blot analysis of extracts of various cell lines using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:1000.

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Polyclonal Antibody

Immunohistochemistry of paraffin-embedded Rat testis using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse testis using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:200 (40x lens).

5 images

Independent Validation

1 validation

产品编号 ABIN7260402

200 μL (More quantities available)

发货至: 中国

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Independently Validated

CD84 抗体 (Internal Region)

CD84 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD84 (CD84) (Internal Region) antibody (ABIN3187786)

Immunohistochemistry validation image for anti-CD84 (CD84) (Internal Region) antibody (ABIN3187786)

Western Blotting (WB) image for anti-CD84 (CD84) (Internal Region) antibody (ABIN3187786)

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

5 images

Independent Validation

1 validation

产品编号 ABIN3187786

100 μL (More quantities available)

发货至: 中国

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Independently Validated

SMAD4 抗体 (N-Term)

SMAD4 适用: 人 WB, IP, CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody (ABIN6972727)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody (ABIN6972727)

SMAD4 antibody (pAb) tested by Western blot.

SMAD4 antibody (pAb) tested by Immunoprecipitation.

4 images

1 reference

Independent Validation

1 validation

产品编号 ABIN6972727

100 μL

发货至: 中国

获取报价

Independently Validated

S1PR1 抗体 (C-Term)

S1PR1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (C-Term) antibody (ABIN2857018)

WB Image EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1, antibody at 1:500 dilution.

WB Image Sample(30 μg of whole cell lysate) A:Hep G2 , 12% SDS PAGE antibody diluted at 1:1000

ICC/IF Image EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.

5 images

Independent Validation

1 validation

产品编号 ABIN2857018

100 μL

发货至: 中国

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Independently Validated

TCF7 抗体 (N-Term)

TCF7 适用: 人 WB, IHC, IP, CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7) (N-Term) antibody (ABIN5620945)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7) (N-Term) antibody (ABIN5620945)

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

4 images

1 reference

Independent Validation

1 validation

产品编号 ABIN5620945

100 μL

发货至: 中国

获取报价

Independently Validated

GAPDH 抗体 (AA 1-335)

GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts of various cell lines, using GAPDH antibody.

Immunohistochemistry of paraffin-embedded mouse lung using GAPDH Rabbit pAb (ABIN3020537, ABIN3020538 and ABIN3020539) at dilution of 1:100 (40x lens).

Immunohistochemistry (IHC) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody (ABIN3020538)

Immunohistochemistry of paraffin-embedded rat pancreas using GAPDH antibody.

Immunohistochemistry of paraffin-embedded human breast cancer using GAPDH antibody.

Immunofluorescence analysis of MCF-7 cells using GAPDH antibody.

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody.

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody.

Tmod1 and Lmod3 Expression in Lmod2 mutant mice.

The effect of different concentrations of Ghrelin on collagen I, collagen III, and PPAR-γ protein expression in Ang II-infused rats.

Ghrelin lowered systolic blood pressure and collagen I and collagen III protein expression in the myocardium of Ang II-infused rats.

Ghrelin attenuated TGF-β1 and TGF-β1 signaling in Ang II-stimulated cardiac fibroblasts.

12 images

13 references

产品编号 ABIN3020538

100 μL (More quantities available)

发货至: 中国

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Caspase 8 抗体 (AA 1-479)

CASP8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Western blot

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase-8 Rabbit pAb (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at dilution of 1:200 (40x lens).

IHC

IHC

Apoptosis related proteins were detected in EOMA cells after treated with DHTS and propranolol.

Western blot analysis of extracts of Jurkat cells, using Caspase-8 antibody (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-8 Rabbit pAb (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using Caspase-8 Rabbit pAb (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit pAb (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HepG2 cells using Caspase-8 Rabbit pAb (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (ABIN6131530, ABIN6137970, ABIN6137972 and ABIN6213676) at 1:1000 dilution.

12 images

17 references

产品编号 ABIN6137970

100 μL (More quantities available)

发货至: 中国

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Lamin A/C 抗体 (AA 403-572)

LMNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts of various cell lines, using LMNA antibody.

Immunohistochemistry (IHC) image for anti-Lamin A/C (LMNA) (AA 403-572) antibody (ABIN3020785)

Immunohistochemistry of paraffin-embedded mouse brain using LMNA antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Human gastric using LMNA antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Human kidney using LMNA antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Human colon using LMNA antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded rat brain using LMNA antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat heart using LMNA antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Human gastric cancer using LMNA antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Human esophageal using LMNA antibody at dilution of 1:100 (x400 lens).

Immunofluorescence (IF) image for anti-Lamin A/C (LMNA) (AA 403-572) antibody (ABIN3020785)

Immunohistochemistry of paraffin-embedded mouse kidney using LMNA antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry (IHC) image for anti-Lamin A/C (LMNA) (AA 403-572) antibody (ABIN3020785)

Immunohistochemistry of paraffin-embedded Human liver injury using LMNA antibody at dilution of 1:100 (x400 lens).

Immunofluorescence analysis of HeLa cell using LMNA antibody.

Immunohistochemistry (IHC) image for anti-Lamin A/C (LMNA) (AA 403-572) antibody (ABIN3020785)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human colon using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human esophageal using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human gastric using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human kidney using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human kidney cancer using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Human liver injury using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded human liver cancer using Lamin A/C antibody.

Immunohistochemistry of paraffin-embedded Rat heart using Lamin A/C antibody.

Immunofluorescence analysis of HeLa cells using Lamin A/C antibody.

32 images

11 references

产品编号 ABIN3020785

100 μL (More quantities available)

发货至: 中国

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