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S1PR1 抗体 (C-Term)

Name: S1PR1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2857018
Availability: OutOfStock
Rating: 5 (1 reviews)

S1PR1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (C-Term) antibody (ABIN2857018)

WB Image EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1, antibody at 1:500 dilution.

IHC

WB Image Sample(30 μg of whole cell lysate) A:Hep G2 , 12% SDS PAGE antibody diluted at 1:1000

ICC/IF Image EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.

5 images

(1 validation)

产品编号 ABIN2857018

发货至: 中国

Datasheet as PDF

抗原 See all S1PR1 抗体

S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))
抗原表位
15

11

8

7

6

5

5

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

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C-Term
适用
92

59

45

8

4

3

3

3

2

1

1

1
人
宿主
90

8
兔
Compatible Secondaries
克隆类型
81

17
多克隆
标记
51

6

6

5

5

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This S1PR1 antibody is un-conjugated
应用范围
75

43

14

14

14

13

12

8

3

3

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to EDG1 (sphingosine-1-phosphate receptor 1)
EDG1 antibody [C2C3], C-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.

亚型

IgG
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 51-150) antibody
S1PR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 311-382) antibody
S1PR1 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-47) antibody
S1PR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E12 unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) antibody
S1PR1 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 1E3H5 unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-47) antibody
S1PR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F11 unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (N-Term) antibody
S1PR1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (Internal Region) antibody
S1PR1 适用: 人, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (Ser311) antibody
S1PR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-382) antibody
S1PR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 17-30), (Extracellular), (N-Term) antibody
S1PR1 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ICC, LCI 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HepG2

限制

仅限研究用
生效 #100014 (Immunofluorescence)

by

Memorial Sloan-Kettering Cancer Center, Parada lab

No.

#100014

日期

2016.05.11

抗原

Sphingosine-1-Phosphate Receptor 1 (S1PR1)

Lot Number

39471

Method validated

Immunofluorescence

Positive Control

Wild-type mouse adult brain cryo-sections

Negative Control

Rabbit IgG

Notes

The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validation Images

IF validation of S1PR1 antibody ABIN2857018 on mouse brain sections. A. ABIN2857018 diluted 1:200 (red), DAPI counterstaining (blue). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.
Full Methods
Primary Antibody

ABIN2857018

Secondary Antibody

Donkey-anti-Rabbit-Cy3

Full Protocol

Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
Immunostaining for Cryo Sections:
Dry slides at 50-55°C for 15-30min (or RT for 30min).
Wash with PBS 5min X 3 (shake gently).
Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
Incubate with ABIN2857018 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
Wash with PBS 5min X 3.
Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
Wash with PBS 5min X 3.
Add three drops of water-based Immu-mount.
Cover with 24 X 60 cover glass.
Imaging:
Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Experimental Notes

ABIN2857018 worked the best at 1:200. At 1:400, or 1:800, it seems to lose some signals in some cells.
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

别名

sphingosine-1-phosphate receptor 1 (S1PR1 产品)

别名

CD363 antibody, CHEDG1 antibody, D1S3362 antibody, ECGF1 antibody, EDG-1 antibody, EDG1 antibody, S1P1 antibody, AI849002 antibody, Edg1 antibody, Lpb1 antibody, S1p antibody, S1p1 antibody, S1P receptor 1 antibody, S1P receptor Edg-1 antibody, edg1 antibody, etID43307.22 antibody, s1p1 antibody, wu:fb98f05 antibody, s1pr1 antibody, xts1p1 antibody, sphingosine-1-phosphate receptor 1 antibody, sphingosine-1-phosphate receptor 1 L homeolog antibody, S1PR1 antibody, S1pr1 antibody, s1pr1 antibody, s1pr1.L antibody

背景

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.

Cellular Localization: Cell membrane, Multi-pass membrane protein

分子量

43 kDa

基因ID

1901

UniProt

P21453

途径

Signaling Events mediated by VEGFR1 and VEGFR2