CD84 抗体 (Internal Region)
(1 validation)-
- 抗原 See all CD84 抗体
- CD84
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD84 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- CD84 Polyclonal Antibody detects endogenous levels of CD84 protein.
- 产品特性
- Rabbit Polyclonal to CD84.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 免疫原
- Synthesized peptide derived from the Internal region of human CD84.
- 亚型
- IgG
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anti-CD84 (CD84) antibody (FITC)
CD84 适用: 人 FACS 宿主: 小鼠 Monoclonal CD84-1-21 FITC
anti-CD84 (CD84) (AA 22-225) antibodyCD84 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 5D12H10 unconjugated
anti-CD84 (CD84) (AA 22-225) antibodyCD84 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 2D11D1 unconjugated
anti-CD84 (CD84) (AA 231-329) antibodyCD84 适用: 小鼠, 大鼠 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD84 (CD84) (AA 22-121) antibodyCD84 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G10 unconjugated
anti-CD84 (CD84) antibodyCD84 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal CD84-1-21 unconjugated
anti-CD84 (CD84) antibodyCD84 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal CD84-1-21 unconjugated
anti-CD84 (CD84) (AA 22-225) antibodyCD84 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD84 (CD84) antibodyCD84 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal 152-1D5 unconjugated
anti-CD84 (CD84) (AA 1-328) antibodyCD84 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000,
- 限制
- 仅限研究用
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- by
- TRON
- No.
- #104537
- 日期
- 2023.12.07
- 抗原
- CD84
- Lot Number
- 15601601
- Method validated
- Immunohistochemistry
- Positive Control
FFPE human tonsil
- Negative Control
- Notes
Passed. The CD84 antibody ABIN3187786 specifically stains cells within the germinal zone in human tonsil.
- Primary Antibody
- ABIN3187786
- Secondary Antibody
- HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)
- Full Protocol
- Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
- xylene 2x for 5 min.
- 100 % (v/v) ethanol 2x for 5 min.
- 96 % (v/v) ethanol 2x for 5 min.
- 70 % (v/v) ethanol 2x for 5 min.
- Wash slides 2x 5 min with ddH2O.
- Cook slides for 30 min at 95 °C in citrate buffer pH 6.
- Wash slides 2x 3 min with ddH2O.
- Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
- Wash slides 3x 3 min in 1X PBS.
- Block slides in 10 % goat serum in PBS for 30 min at RT.
- Incubate slides with primary rabbit anti-CD84 antibody (antibodies-online, ABIN3187786, lot 15601601) diluted 1:100 and 1:200 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
- Wash slides 3x 3 min in 1X PBS.
- Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
- Wash slides 3x 3 min in 1X PBS.
- HRP development.
- Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
- Wash slides for 15 min under running tap water.
- Dehydrate sections in an ascending alcohol-xylene series:
- 70% ethanol 2x for 10 sec.
- 96% ethanol 2x for 10 sec.
- 100% ethanol 2x for 10 sec.
- xylene 2x for 10 sec.
- Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
- Dry sections.
- Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.
- Experimental Notes
Staining with ABIN3187786 overnight at 1:100 and 1:200 antibody dilution the signal in human tonsil was sharp and localized in the cells within the germinal zone (as expected). Modifying ABIN3187786 incubation conditions to 1 h at RT, the signal in human tonsil was still sharp and localized in the cells within the germinal zone.
However, in mouse spleen, the signal was almost absent and very weak. With the modified incubation conditions, the signal in mouse spleen was again almost absent and very weak. With either staining protocol the signal in mouse lung was sporadic and its quality does not allow the possibility of non-specific staining to be excluded.
生效 #104537 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IHC staining of human tonsil with anti-CD84 antibody ABIN3187786 (1:100) and counterstain with hematoxylin.]()
IHC staining of human tonsil with anti-CD84 antibody ABIN3187786 (1:100) and counterstain with hematoxylin.
![IHC staining of human tonsil with anti-PD-1 antibody ABIN3187786 (1:200) and counterstain with hematoxylin.]()
IHC staining of human tonsil with anti-PD-1 antibody ABIN3187786 (1:200) and counterstain with hematoxylin.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C.
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