BRAF 抗体
(2 references)Quick Overview for BRAF 抗体 (ABIN968991)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- BRAF Antibody
-
纯化方法
- Ascitic fluid
-
免疫原
- Purified recombinant fragment of human BRAF expressed in E. Coli.
-
亚型
- IgG1
-
-
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibody
BRAF 适用: 人, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C11 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A9 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) antibodyBRAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3D2 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1H12F1 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 424-453) antibodyBRAF 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, IF 宿主: 小鼠 Monoclonal 5C5 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB22681 unconjugated
-
-
应用备注
-
ELISA: 1/10000
ICC: 1/200 - 1/1000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Ascitic fluid containing 0.03 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
-
-
-
: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, (2008) (PubMed).
: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, (2006) (PubMed).
-
: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, (2008) (PubMed).
-
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
-
别名
- BRAF
-
背景
- BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
-
分子量
- 87 kDa
-
基因ID
- 673
-
UniProt
- P15056
-
途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
-
