PSME3 抗体 (AA 45-147)
(1 reference)-
- 抗原 See all PSME3 抗体
- PSME3
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抗原表位
- AA 45-147
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适用
- 人, 大鼠, 小鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PSME3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), BioImaging (BI)
- 交叉反应
- 大鼠, 犬, 人
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Psme3/PA28-gamma aa. 45-147
- 克隆位点
- 47-Psme3
- 亚型
- IgG1
- Top Product
- Discover our top product PSME3 Primary Antibody
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anti-PSME3 (PSME3) (AA 7-254) antibody
PSME3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) antibodyKO Validated PSME3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (AA 2-252) antibodyPSME3 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) antibodyPSME3 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (AA 1-254) antibodyPSME3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-PSME3 (PSME3) (C-Term) antibodyPSME3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (N-Term) antibodyPSME3 适用: 人, 大鼠, 小鼠, 犬, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (AA 1-254) antibodyPSME3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (N-Term) antibodyPSME3 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-PSME3 (PSME3) (AA 48-97) antibodyPSME3 适用: 人, 大鼠, 小鼠, 犬, Pig, Cow, 豚鼠, 马, 兔, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - 说明
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Related Products: ABIN967389, ABIN968545
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4923-35, (1998) (PubMed).
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: "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4923-35, (1998) (PubMed).
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- 抗原
- PSME3
- 别名
- Psme3 (PSME3 产品)
- 别名
- AA410043 antibody, AU020960 antibody, Ki antibody, PA28gamma antibody, REGgamma antibody, pa28g antibody, Ab2-371 antibody, PA28-gamma antibody, PA28G antibody, REG-GAMMA antibody, psme3b antibody, proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antibody, proteasome activator subunit 3 antibody, proteasome activator subunit 3 S homeolog antibody, Psme3 antibody, PSME3 antibody, psme3.S antibody
- 背景
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The 20S proteasome is the major constituent of the proteasomal complex that mediates degradation events, including the generation of antigenic peptides that interact with MHC class I. The 20S proteasome is a cylindrical shaped complex composed of four layers of rings, each with seven subunits. The inner rings contain alpha-subunits, while the outer rings contain beta-catalytic subunits. The constituents of the PA28 activator complex are additional subunits with specialized roles in class I-mediated antigen presentation. PA28 is a ring-shaped structure with alternating alpha- and beta-subunits. This complex binds the alpha-rings of 20S and stimulates its activity. Expression of the PA28 alpha- and beta-subunits is strongly induced by IFN-gamma. The protein product of the Psme3 gene, also known as the Ki antigen, is highly homologous to the PA28 alpha- and beta-subunits and has been designated the PA28 gamma-subunit. This protein forms a homohexamer that binds the 20S proteasome and is thought to modulate proteasome activity. The PA28 alpha- and beta-subunits are located in the cytoplasm and in the nucleus, while the gamma-subunit is almost exclusively nuclear.
Synonyms: PA28-gamma - 分子量
- 36 kDa
- 途径
- Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA
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