CENPF 抗体 (AA 209-381)
(5 references)Quick Overview for CENPF 抗体 (AA 209-381) (ABIN968167)
抗原
See all CENPF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 209-381
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Mitosin aa. 209-381
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亚型
- IgG1
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anti-Centromere Protein F (CENPF) (C-Term) antibody
CENPF 适用: 人 WB, IHC (p), IF, ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 436-637) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyCENPF 适用: 人, 小鼠 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 551-650) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyVerified CENPF 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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说明
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Related Products: ABIN968537, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, (2003) (PubMed).
: "Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies." in: Cytometry, Vol. 25, Issue 1, pp. 90-8, (1997) (PubMed).
: "Autoimmunity to the cell cycle-dependent centromere protein p330d/CENP-F in disorders associated with cell proliferation." in: Journal of autoimmunity, Vol. 8, Issue 4, pp. 575-86, (1996) (PubMed).
: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, (1995) (PubMed).
: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, (1994) (PubMed).
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: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, (2003) (PubMed).
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- CENPF (Centromere Protein F (CENPF))
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别名
- Mitosin
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背景
- Mitosin, a nuclear proetin of 3113 amino acids, contains a pair of tandem repeats and seven leucine repeats. In vitro, Mitosin directly interacts with the retinoblastoma protein, Rb. Mitosin is expressed throughout the cell cycle, with levels being lowest during G1, and it localizes at the kinetochore during the mitotic phase. The subcellular redistribution of Mitosin to the kinetochore is linked to its phosphorylation. Its expression is linked to patients with autoimmune diseases characterized by abnormal cell proliferation. Ectopic expression of a truncated version of Mitosin blocks the progression of the cell cycle. This suggests that the protein has an important role during cell proliferation. Because of similarities in subcellular localization and expression, Mitosin and the autoantigen p300/CENP-F, which is also linked to a multitude of autoimmune disorders, are probably the same protein.
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分子量
- 357 kDa
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途径
- Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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