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BAD 抗体 (AA 39-198)

Name: BAD 抗体 (AA 39-198)
SKU: ABIN967940
Availability: OutOfStock

This anti-BAD antibody is a 小鼠单克隆 antibody detecting BAD in WB, IHC, IF 和 IP. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of Bad on an A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). (BAD 抗体 (AA 39-198))

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75). (BAD 抗体 (AA 39-198))

2 images

(4 references)

产品编号 ABIN967940

发货至: 中国

Datasheet as PDF

Quick Overview for BAD 抗体 (AA 39-198) (ABIN967940)

抗原

See all BAD 抗体

BAD (BCL2-Associated Agonist of Cell Death (BAD))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This BAD antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

48-Bad

Discover our top product BAD Primary Antibody

抗原表位
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23

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22

15

8

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8

8

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2

2

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2

2

2

2

1

1

1

1
AA 39-198

交叉反应

人, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Bad aa. 39-198

亚型

IgG2b
anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 101-204) antibody
BAD 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
BAD 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
BAD 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1G5B3 unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 69-168) antibody
BAD 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3H8 unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer136) antibody
BAD 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer155) antibody
BAD 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 92-127) antibody
BAD 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody
BAD 适用: 人 ELISA, IHC, DB, IF 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
BAD 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 53-81) antibody
BAD 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB06939 unconjugated

说明

Related Products: ABIN968533, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Tomicic, Thust, Kaina: "Ganciclovir-induced apoptosis in HSV-1 thymidine kinase expressing cells: critical role of DNA breaks, Bcl-2 decline and caspase-9 activation." in: Oncogene, Vol. 21, Issue 14, pp. 2141-53, (2002) (PubMed).

Walsh, Lutz, Cotter, OConnor: "Erythrocyte survival is promoted by plasma and suppressed by a Bak-derived BH3 peptide that interacts with membrane-associated Bcl-X(L)." in: Blood, Vol. 99, Issue 9, pp. 3439-48, (2002) (PubMed).

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, (2001) (PubMed).

Graff, Konicek, McNulty, Wang, Houck, Allen, Paul, Hbaiu, Goode, Sandusky, Vessella, Neubauer: "Increased AKT activity contributes to prostate cancer progression by dramatically accelerating prostate tumor growth and diminishing p27Kip1 expression." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24500-5, (2000) (PubMed).
抗原

BAD (BCL2-Associated Agonist of Cell Death (BAD))

别名

Bad

背景

Isolated by screening for Bcl-2 interacting proteins, Bad shows significant homology to Bcl-2 within the Bcl-2 homology domains 1 and 2 (BH1 and BH2). In addition, several other proteins involved in cell death such as Bax, Bcl-X[L], Mcl-1, and A1 share similar homology with Bcl-2. Bcl-2 is known to oppose several apoptotic signals and is considered to be a central downstream cell death repressor. Bcl-X[L] represses apoptosis, but its short form, Bcl-X[S], promotes cell death. Bax is known to homodimerize as well as heterodimerize with Bcl-2. An excess concentration of Bax opposes the ability of Bcl-2 to repress cell death. Bad can selectively dimerize with Bcl-X[L] and Bcl-2, but not with Bax, Bcl-X[S], Mcl-1, A1, or itself. In mammalian cells, Bad binds more strongly to Bcl-X[L] than Bcl-2. This may explain why Bad reverses the death repressor activity of Bcl-X[L], but not that of Bcl-2. The formation of the Bad-Bcl-X[L] heterodimer displaces Bax and restores favorable conditions for apoptosis. This antibody is tested by western blot analysis.

分子量

23 kDa

途径

MAPK Pathway, PI3K-Akt Signaling, RTK signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events