MEK1 抗体 (pSer298)
(3 references)Quick Overview for MEK1 抗体 (pSer298) (ABIN967599)
抗原
See all MEK1 (MAP2K1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer298
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品牌
- BD Pharmingen™
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交叉反应
- 小鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Phosphorylated Human MEK1 Peptide
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亚型
- IgG1 kappa
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 适用: 人 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibodyMAP2K1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (C-Term) antibodyMAP2K1 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibodyMAP2K1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 54-369) antibodyMAP2K1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-79) antibodyMAP2K1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 3F10 unconjugated Recombinant Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, (2003) (PubMed).
: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, (2002) (PubMed).
: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, (2001) (PubMed).
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: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, (2003) (PubMed).
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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别名
- MEK1
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背景
- MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.
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分子量
- 45 kDa
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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