IL-6 抗体
Quick Overview for IL-6 抗体 (ABIN964780)
抗原
See all IL-6 (IL6) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 210-401-310
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Supplier
- Rockland
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原理
- IL-6 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse IL-6 protein.
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产品特性
- Synonyms: rabbit anti-IL-6 antibody, rabbit anti-Interleukin-6 antibody, Interleukin6 cytokine, IL6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Interleukin 6 (IL6) (AA 111-150) antibody
IL6 适用: 人, 小鼠, 大鼠, 兔, Cow WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) antibodyIL6 适用: 人 ELISA, FACS, Func, ELISpot 宿主: 小鼠 Monoclonal B-E8 unconjugated
anti-Interleukin 6 (IL6) (AA 21-211) antibodyIL6 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 6 (IL6) (AA 27-212) antibodyIL6 适用: 猕猴 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 18# unconjugated
anti-Interleukin 6 (IL6) (AA 21-211) antibodyIL6 适用: 大鼠 WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 6 (IL6) (AA 101-150) antibodyIL6 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 27-241) antibodyIL6 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C506 unconjugated
anti-Interleukin 6 (IL6) (AA 25-211) antibodyIL6 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 27-55) antibodyIL6 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 6 (IL6) (AA 110-185) antibodyIL6 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This purified antibody has been tested for use in western blotting against recombinant mouse IL-6 spiked into cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysates or extracts.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store anti-IL-6 at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 6 months
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- IL-6 (IL6) (Interleukin 6 (IL6))
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别名
- Il6
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背景
- Background: Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
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基因ID
- 16193
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NCBI登录号
- NP_112445
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UniProt
- P08505
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途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
抗原
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