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Collagen IV 抗体

The 兔 多克隆 anti-Collagen IV antibody is suitable to detect Collagen IV in samples from 人. It has been validated for ELISA, IHC, IHC (fro), IHC (p) 和 IF.
产品编号 ABIN961130
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Collagen IV 抗体 (ABIN961130)

抗原

See all Collagen IV (COL4) 抗体
Collagen IV (COL4) (Collagen, Type IV (COL4))

适用

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宿主

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克隆类型

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多克隆

标记

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This Collagen IV antibody is un-conjugated

应用范围

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ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
  • 特异性

    Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.

    纯化方法

    Affinity purified

    免疫原

    Human placental collagen type IV.

    Type of Immunogen: Purified protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.

    Not recommended for: WB

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, mannitol, dextran, no preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Collagen IV (COL4) (Collagen, Type IV (COL4))

    别名

    Collagen IV
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