Collagen IV 抗体
Quick Overview for Collagen IV 抗体 (ABIN961130)
抗原
See all Collagen IV (COL4) 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.
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纯化方法
- Affinity purified
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免疫原
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Human placental collagen type IV.
Type of Immunogen: Purified protein -
亚型
- IgG
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.
Not recommended for: WB -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, mannitol, dextran, no preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- Collagen IV
抗原
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