Amylase beta 抗体
amyB
适用: Sweet potato
WB, ELISA
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all Amylase beta (amyB) products
- Amylase beta (amyB)
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适用
- Sweet potato
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Amylase beta antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.
- 纯度
- Anti-BETA AMYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b Amylase [Sweet Potato].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Amylase beta (amyB) antibody (Biotin)
amyB 适用: Sweet potato WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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BETA AMYLASE antibody has been assayed against 1.0 µg of b Amylase [Sweet Potato] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:16.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Amylase beta (amyB)
- 别名
- BETA AMYLASE (amyB 产品)
- 别名
- beta-amylase antibody, Beta-amylase antibody, CBO1203 antibody, amyB antibody, CLM_1356 antibody, BMQ_0356 antibody, BMD_0357 antibody
- 背景
- Synonyms: 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
- UniProt
- P10537
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