Amylase beta 抗体 (HRP)
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- 抗原 See all Amylase beta (amyB) products
- Amylase beta (amyB)
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适用
- Sweet potato
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Amylase beta antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Beta Amylase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].
- 免疫原
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Immunogen: Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Amylase beta (amyB) antibody (Biotin)
amyB 适用: Sweet potato WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Application Note: Anti-Beta Amylase has been assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:40,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Amylase beta (amyB)
- 别名
- Beta Amylase (amyB 产品)
- 别名
- beta-amylase antibody, Beta-amylase antibody, CBO1203 antibody, amyB antibody, CLM_1356 antibody, BMQ_0356 antibody, BMD_0357 antibody
- 背景
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Synonyms: 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.
Gene Name: BMY1, AMYB
- UniProt
- P10537
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