RAG1 抗体 (AA 351-450) (Alexa Fluor 350)
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- 抗原 See all RAG1 抗体
- RAG1 (Recombination Activating Gene 1 (RAG1))
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抗原表位
- AA 351-450
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAG1 antibody is conjugated to Alexa Fluor 350
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- 交叉反应
- 小鼠
- 预测反应
- Human,Rat,Sheep,Pig
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human RAG1/RNF74
- 亚型
- IgG
- Top Product
- Discover our top product RAG1 Primary Antibody
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anti-Recombination Activating Gene 1 (RAG1) (AA 818-868) antibody
RAG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-Recombination Activating Gene 1 (RAG1) antibodyRAG1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 1 (RAG1) (AA 946-995) antibodyRAG1 适用: 小鼠, 人, Pig, 大鼠, 小鸡, 兔, 非洲爪蟾, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 1 (RAG1) (AA 1-270) antibodyRAG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RAG1 (Recombination Activating Gene 1 (RAG1))
- 别名
- RNF74 (RAG1 产品)
- 别名
- RAG-1 antibody, rag1 antibody, RNF74 antibody, Rag-1 antibody, rag-1 antibody, rag1a antibody, recombination activating 1 antibody, recombination activating gene 1 antibody, recombination activating gene 1 S homeolog antibody, Rag1 antibody, RAG1 antibody, rag1 antibody, rag1.S antibody
- 背景
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Synonyms: RAG-1, RNF74, V(D)J recombination-activating protein 1, RING finger protein 74, RAG1
Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
- 基因ID
- 5896
- UniProt
- P15918
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