Recognizes human mast cell tryptase, both alpha and beta isoforms. Crossreacts with dog, cat and monkey. Does not bind to any other cell type in immunohistochemistry.
产品特性
Monoclonal antibody to Human Mast Cell Tryptase, alpha and beta isoforms
纯化方法
Protein G Chromatography
免疫原
Human mast cell tryptase purified from human lung tissue.
Suitable for use in Immunohistochemistry (paraffin sections at 1:10.000 and frozen sections), ELISA and Western blot. IHC paraffin sections require antigen retrieval using pronase or heat/sodium citrate buffer pH 6.0. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Prepare working dilution only prior to immediate use.
限制
仅限研究用
状态
Liquid
缓冲液
PBS
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Short term store at 2-8 °C. Long term, store at -28 °C.