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BrdU 抗体

BrdU FACS, IHC (fro) 宿主: 小鼠 Monoclonal 131-14871 (AH4H7-1) unconjugated
产品编号 ABIN782023
发货至: 中国
  • 抗原 See all BrdU 抗体
    BrdU (Bromodeoxyuridine (BrdU))
    适用
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    宿主
    • 34
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    小鼠
    克隆类型
    • 42
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    单克隆
    标记
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    This BrdU antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    纯化方法
    Protein A Chromatography
    克隆位点
    131-14871 (AH4H7-1)
    亚型
    IgG1
    Top Product
    Discover our top product BrdU Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL (OD280nm, E0.1% = 1.4)
    缓冲液
    10 mM Tris, pH 7.5 containing 150 mM Sodium Chloride and 0.09 % Sodium Azide as preservative
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    BrdU (Bromodeoxyuridine (BrdU))
    Abstract
    BrdU 产品
    物质类
    Chemical
    背景
    Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
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