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BAD 抗体 (pSer118)

Name: BAD 抗体 (pSer118)
SKU: ABIN756562
Availability: OutOfStock

BAD 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

2 images

产品编号 ABIN756562

发货至: 中国

Datasheet as PDF

抗原 See all BAD 抗体

BAD (BCL2-Associated Agonist of Cell Death (BAD))
抗原表位
34

34

27

26

26

24

24

23

15

8

8

8

8

8

8

8

8

7

7

7

7

7

7

7

7

7

7

7

7

7

7

6

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1
pSer118
适用
374

218

140

17

9

6

4

3

3

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
445

15

1
兔
Compatible Secondaries
克隆类型
427

34
多克隆
标记
178

41

37

35

34

31

11

11

11

11

11

11

6

6

6

6

6

2

2

2

1

1

1
This BAD antibody is un-conjugated
应用范围
252

242

163

95

80

79

56

54

21

19

12

10

9

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

KO-Validated

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser118

亚型

IgG

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anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
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anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
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anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 69-168) antibody
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anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer155) antibody
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anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer136) antibody
BAD 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody
BAD 适用: 人 ELISA, IHC, DB, IF 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 92-127) antibody
BAD 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 1-168) antibody
BAD 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 100-130) antibody
BAD 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

BAD (BCL2-Associated Agonist of Cell Death (BAD))

别名

Bad (BAD 产品)

别名

bad antibody, fa01b12 antibody, wu:fa01b12 antibody, wu:fa96d04 antibody, BAD antibody, BBC2 antibody, BCL2L8 antibody, AI325008 antibody, Bbc2 antibody, BCL2-associated agonist of cell death b antibody, BCL2 associated agonist of cell death antibody, BCL2-associated agonist of cell death antibody, badb antibody, BAD antibody, Bad antibody

背景

Synonyms: Bad phospho S118, Bad phospho Ser118, p-Bad S118,p- Bad Ser118, p-Bad phospho Ser118, BBC 2, BBC2, BBC6, Bcl 2 Antagonist of Cell Death, Bcl 2 Binding Component 6, BCL X / BCL 2 Binding Protein, mouse BAD Ser155, rat BAD Ser156
Background: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

基因ID

572

UniProt

Q92934

途径

MAPK Pathway, PI3K-Akt Signaling, RTK signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events