LRP2 抗体 (AA 3401-3500) (Cy3)
(1 validation)Quick Overview for LRP2 抗体 (AA 3401-3500) (Cy3) (ABIN750991)
抗原
See all LRP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 3401-3500
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交叉反应
- 人, 小鼠
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预测反应
- Rat,Dog,Horse,Chicken,Rabbit
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human Megalin
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亚型
- IgG
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anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody
LRP2 适用: 人, 小鼠 ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 202-332) antibodyLRP2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 4035-4184) antibodyLRP2 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) antibodyLRP2 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 4446-4655) antibodyLRP2 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 1211-1369) antibodyLRP2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) antibodyLRP2 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (Internal Region) antibodyLRP2 适用: 人, 小鼠, 大鼠 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 4626-4655) antibodyLRP2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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- by
- CaresBio Laboratory
- No.
- #029611
- 日期
- 2014.02.11
- 抗原
- Lot Number
- YEYY9
- Method validated
- Immunofluorescence
- Positive Control
- MCF7 cells
- Negative Control
- HeLa cells
- Notes
- Strong signal was detected in positive control tissues and not in negative control tissues. Note that there was a small amount of signal generated in the negative control sample.
- Primary Antibody
- Antigen: Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) antibody (Cy3)
- Catalog number: ABIN750991
- Lot number: YEYY9
- Secondary Antibody
- Used only for the secondary only control, as primary antibody was directly conjugated to Cy3
- Antibody: Cy3 Goat Anti-Rabbit IgG (H+L)
- Full Protocol
- MCF7 and HeLa cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
- Fixed cells were rinsed three times in PBS for 5 min each at RT.
- Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
- Cells were incubated with fluorophore conjugated primary antibody diluted 1:250 and 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
- Isotype control staining:
- All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Cells were incubated with goat anti rabbit CY3 conjugated secondary antibody for 60 min in dark at RT.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI. Secondary only staining:
- All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with goat anti rabbit CY3 conjugated secondary antibody for 60 min in dark at RT.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
- Stained cells were imaged with a Nikon C2+ confocal microscope.
- Experimental Notes
- We did the first set of experiment with 1:250 dilution of the primary antibody but have observed lower level of expression of LRP2 in MCF7 (positive) cells (Figure 2). We repeated experiment with higher concentration of the primary antibody (1:100) and the expression level increased as shown in Figure 1. We have noticed lower level of expression of LRP2 in HeLa (negative) cells (Figure 3). We have used rabbit normal serum for isotype control to match the species with primary antibody (rabbit) and also we used bovine albumin serum (BSA) as blocking and antibody diluent buffer to reduce species cross reactivity.
生效 #029611 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: MCF7 cells stained at 1:250 with LRP2 (red) and a nuclear counterstain (blue).]()
Figure 1: MCF7 cells stained at 1:250 with LRP2 (red) and a nuclear counterstain (blue).
![Figure 2: MCF7 cells stained at 1:100 with LRP2 (red) and a nuclear counterstain (blue).]()
Figure 2: MCF7 cells stained at 1:100 with LRP2 (red) and a nuclear counterstain (blue).
![Figure 3: HeLa cells stained with LRP2 (red) and a nuclear counterstain (blue).]()
Figure 3: HeLa cells stained with LRP2 (red) and a nuclear counterstain (blue).
![Figure 4: MCF7 cells stained isotype control (red) and a nuclear counterstain (blue).]()
Figure 4: MCF7 cells stained isotype control (red) and a nuclear counterstain (blue).
![Figure 1: MCF7 cells with only a secondary antibody (red) and a nuclear counterstain (blue).]()
Figure 1: MCF7 cells with only a secondary antibody (red) and a nuclear counterstain (blue).
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- LRP2 (Low Density Lipoprotein Receptor-Related Protein 2 (LRP2))
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别名
- Lrp2/Megalin
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背景
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Synonyms: DBS, GP33, Low-density lipoprotein receptor-related protein 2, LRP-2, Glycoprotein 33, Megalin, LRP2
Background: Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
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基因ID
- 4036
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UniProt
- P98164
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途径
- Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport
抗原
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