RAD51 抗体 (AA 1-134)
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北京 101111
Quick Overview for RAD51 抗体 (AA 1-134) (ABIN7868113)
抗原
See all RAD51 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-134
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原理
- RAD51 Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- A portion of amino acids 1-134 from the human protein was used as the immunogen for this RAD51 antibody.
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亚型
- IgG2b, kappa
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应用备注
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The stated application concentrations are suggested starting points. Titration of the RAD51 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the RAD51 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- RAD51 (DNA Repair Protein Homolog 1 (RAD51))
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别名
- RAD51
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背景
- The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]
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UniProt
- Q06609
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途径
- DNA Damage Repair
抗原
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