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RAD51 抗体 (AA 81-180)

Name: RAD51 抗体 (AA 81-180)
SKU: ABIN4913431
Availability: OutOfStock

RAD51 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated at 1:300 overnight at 4˚C. (RAD51 抗体 (AA 81-180))

HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (RAD51 抗体 (AA 81-180))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (BSA or normal goat serum) at 37°C for 20min, Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. (RAD51 抗体 (AA 81-180))

IHC (p)

3 images

(2 references)

产品编号 ABIN4913431

发货至: 中国

Datasheet as PDF

抗原 See all RAD51 抗体

RAD51 (DNA Repair Protein Homolog 1 (RAD51))
抗原表位
18

14

10

8

7

6

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 81-180
适用
123

85

43

4

3

3

3

3

2

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
105

21

1
兔
Compatible Secondaries
克隆类型
98

29
多克隆
标记
71

6

5

5

5

5

5

5

4

3

2

2

2

2

2

2

1
This RAD51 antibody is un-conjugated
应用范围
93

38

28

28

23

23

20

16

10

8

7

4

2

2

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Rad51

亚型

IgG

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RAD51 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-134) antibody
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anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-134) antibody
RAD51 适用: 人 IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal RAD51-2702 unconjugated

anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-242) antibody
RAD51 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E5-E5 unconjugated

anti-DNA Repair Protein Homolog 1 (RAD51) antibody
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anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-242) antibody
RAD51 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4C11-B4 unconjugated

anti-DNA Repair Protein Homolog 1 (RAD51) antibody
RAD51 适用: 人, 小鼠 WB, ELISA, IP, IF 宿主: 小鸡 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Qi, Jiang, Wang, Yang, Li, He, Yu: "Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages." in: Oncotarget, Vol. 8, Issue 17, pp. 29300-29317, (2018) (PubMed).
抗原

RAD51 (DNA Repair Protein Homolog 1 (RAD51))

别名

Rad51 (RAD51 产品)

别名

BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, MRMV2 antibody, RAD51A antibody, RECA antibody, CRAD51 antibody, wu:fb38e12 antibody, zgc:77754 antibody, RGD1563603 antibody, ATRAD51 antibody, F22D1.20 antibody, F22D1_20 antibody, RAS associated with diabetes protein 51 antibody, AV304093 antibody, Rad51a antibody, Reca antibody, brcc5 antibody, rad51 antibody, rad51a antibody, reca antibody, xrad51 antibody, RAD51 recombinase antibody, RAS associated with diabetes protein 51 antibody, RAD51 recombinase L homeolog antibody, RAD51 antibody, rad51 antibody, Rad51 antibody, rad51.L antibody

背景

Synonyms: RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RAD51
Background: Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

基因ID

5888

UniProt

Q06609

途径

DNA Damage Repair