BRAF 抗体 (N-Term)
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Quick Overview for BRAF 抗体 (N-Term) (ABIN7860825)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 596-606, N-Term
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原理
- BRAF (V600E Mutant Specific) (Prognostic Marker) Antibody
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特异性
- TheÇ 79oΩBRAFÇ 79oΩgene encodes a cytoplasmic serine-threonine kinase, which initiates the activation of the mitogen-activated protein kinase (MAPK) signalling pathway. The oncogenic mutations in the kinase region of BRAF gene result in constitutive activation of the MAPK signalling pathway, leading to increased cell proliferation, resistance to apoptosis and tumor progression. The most common of all activating BRAF mutations leads to a substitution of valine (V) to glutamic acid (E) at the position 600 of the amino acid sequence. TheÇ 79oΩBRAFÇ 79oΩV600E mutation is an important predictive and prognostic biomarker. The BRAF V600E mutation is detected in approximately 8 % of all solid tumours, including 45 % of papillary thyroid carcinomas, 40-60 % of melanomas, 5-15 % of colorectal adenocarcinomas, 35 % of serous low grade and borderline ovarian carcinomas, 1-3 % of non-small cell lung cancers, and 5-7 % of cholangiocarcinomas.Ç 79oΩFurthermore, the BRAF V600E mutation is found in 100 % of hairy cell leukaemia,Ç 79oΩ54 % Erdheim-Chester disease, 38 % of Langerhans cell histocytosesÇ 79oΩand 60 % of pleomorphic xanthoastrocytomas.Ç 79oΩ
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- An eleven amino acid residue long synthetic peptide (aa596-606), corresponding to Cys-GLAT(E)KSRWSG from human BRAF protein. It was conjugated to KLH at the N-terminus.
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亚型
- IgG1, kappa
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anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibody
BRAF 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A9 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C11 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) antibodyBRAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3D2 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1H12F1 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 38-230) antibodyBRAF 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 424-453) antibodyBRAF 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, IF 宿主: 小鼠 Monoclonal 5C5 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB22681 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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别名
- BRAF
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背景
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FLJ95109, 94 kDa B raf protein, B Raf proto oncogene, serine/threonine kinase, B RAF1, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF 1, BRAF, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral (v-raf) oncogene homolog B1, NS7, oncogene BRAF1, p94, Proto-oncogene B-Raf, Proto-oncogene c-Rmil, RAFB1, RMIL, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1
A431|Jurkat and HeLa cells. Human melanoma and colon carcinoma, Human Skeletal Muscles
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分子量
- 35kDa (predicted)
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基因ID
- 673, 550061
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UniProt
- P15056
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途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
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