Recombinant MGMT 抗体 (AA 1-100)
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北京 101111
Quick Overview for Recombinant MGMT 抗体 (AA 1-100) (ABIN7864246)
抗原
See all MGMT 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-100
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原理
- Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody
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特异性
- Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossT 79oΩ 79oΩlinks at the O6T 79oΩ 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6T 79oΩ 79oΩposition of guanine in DNA (prior to crossT 79oΩ 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNAT 79oΩ 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
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亚型
- IgG, kappa
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anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 32-210) antibody
MGMT 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A11F8 unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 101-207) antibodyMGMT 适用: 大鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (N-Term) antibodyMGMT 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 156-182), (C-Term) antibodyMGMT 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19082 unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 156-182) antibodyMGMT 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibodyMGMT 适用: 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibodyMGMT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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别名
- MGMT
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背景
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6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase
Jurkat, MCF-7, LNCaP, Hep G2 and human liver. Human tonsil or stomach (IHC).
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分子量
- 21.646 kDa
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基因ID
- 4255, 501522
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UniProt
- P16455
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途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
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