HLA-A 抗体
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北京 101111
Quick Overview for HLA-A 抗体 (ABIN7839650)
抗原
See all HLA-A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody to HLA-A/B/C
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特异性
- Recognizes human HLA-A/B/C
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纯化方法
- The antibody was purified by affinity chromatography.
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anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 1-365) antibody
HLA-A 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 1-365) antibodyHLA-A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 24-365) antibodyHLA-A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D6 unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 82-106), (N-Term) antibodyHLA-A 适用: 人 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal 108-2C5 unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 70-99) antibodyHLA-A 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人, 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
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别名
- HLA-A
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背景
- HLA-A, HLAA, HLA class I histocompatibility antigen, A-68 alpha chain, Aw-68, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A*68, HLA-A, HLAA, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A*2, HLA-A, HLAA, HLA class I histocompatibility antigen, A-3 alpha chain, MHC class I antigen A*3, HLA-A, HLAA, HLA class I histocompatibility antigen, A-24 alpha chain, Aw-24, HLA class I histocompatibility antigen, A-9 alpha chain, MHC class I antigen A*24, HLA-A, HLAA, HLA class I histocompatibility antigen, A-32 alpha chain, MHC class I antigen A*32, HLA-A, HLAA, HLA class I histocompatibility antigen, A-69 alpha chain, Aw-69, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A*69, HLA-A, HLAA, HLA class I histocompatibility antigen, A-11 alpha chain, MHC class I antigen A*11, HLA-A, HLAA, HLA class I histocompatibility antigen, A-31 alpha chain, MHC class I antigen A*31, HLA-A, HLAA, HLA class I histocompatibility antigen, A-25 alpha chain, HLA class I histocompatibility antigen, A-10 alpha chain, MHC class I antigen A*25, HLA-A, HLAA, HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen A*1, HLA-A, HLAA, HLA class I histocompatibility antigen, A-26 alpha chain, MHC class I antigen A*26, HLA-A, HLAA, HLA class I histocompatibility antigen, A-34 alpha chain, Aw-34, HLA class I histocompatibility antigen, A-10 alpha chain, MHC class I antigen A*34, HLA-A, HLAA, HLA class I histocompatibility antigen, A-36 alpha chain, Aw-36, MHC class I antigen A*36
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基因ID
- 3105
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UniProt
- P04439
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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