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TRIM29 抗体 (AA 1-200) (CF®740)

This anti-TRIM29 antibody is a 小鼠 单克隆 antibody detecting TRIM29 in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7661076
发货至: 中国

Quick Overview for TRIM29 抗体 (AA 1-200) (CF®740) (ABIN7661076)

抗原

See all TRIM29 抗体
TRIM29 (Tripartite Motif Containing 29 (TRIM29))

适用

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宿主

  • 36
  • 33
  • 1
小鼠

克隆类型

  • 37
  • 33
单克隆

标记

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This TRIM29 antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

TRIM29-1041
  • 抗原表位

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    AA 1-200

    原理

    TRIM29(TRIM29/1041), CF740 conjugate

    产品特性

    This antibody recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

    亚型

    IgG2a, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: A431 cells. Tonsil or Squamous cell carcinoma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    TRIM29 (Tripartite Motif Containing 29 (TRIM29))

    别名

    TRIM29

    背景

    Synonyms: Ataxia telangiectasia group D complementing gene (ATDC), Tripartite motif-containing protein 29 (TRIM29),

    Gene Symbol: TRIM29

    分子量

    66 kDa

    基因ID

    23650

    UniProt

    Q14134

    途径

    p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
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